Karoo Heartland is in the Eastern Cape of South Africa.
Cities
- Aberdeen — an architectural conservation area with many examples of Victorian architecture
- Cookhouse — Many believe Cookhouse was named after a small stone kitchen erected by garrison troops during the frontier wars. Today best known as a railway junction.
- Cradock — home of the annual Fish River Canoe Marathon, a two-day, 80-km event
- Graaff-Reinet — renowned for its architectural heritage and has more national monuments than any other town in South Africa
- Middelburg — this little burg is in the middle of it all
- Nieu-Bethesda — a remote artistic community known for the "Owl House"
- Somerset East — produces some of the finest roses in the country, and is home to the Walter Battiss Art Gallery
- Steytlerville —
- Tarkastad — Tarkastad mountain is nearby
Other destinations
- Camdeboo National Park — a 194-km² park in the foothills of the Sneeuberge (Snow Mountains), known for the spectacular Valley of Desolation
- Mountain Zebra National Park — home to the very rare and endangered mountain zebra